The biostat rm rocking motion, is a fully gmp compliant, wave mixed bioreactor. To measure ph and do with these sensors, a wavepod ii controller is required for wave bioreactor 2050 system. Applikon ezcontrol bioreactor controller operation before the bioreactor is autoclaved, the ph acidity sensor needs to be calibrated with ph 4. Ge readytoprocess cbcu cell culture and fermentation. Ability to handle the bioreactor in a tilt position facilitates sampling and harvest. For more information on wave bioreactor systems, please see. Perfusion cell culture using wave bioreactor systems 210 and 2050. Process development and optimization using the readytoprocess. The system is based on a wellknown rocking technology that provides mixing and aeration to the culture, which is maintained in an inflated, disposable cellbag bioreactor. The singleuse readytoprocess wave 25 bioreactor system is a reliable and intuitive cell culture device for working volumes in the 0. Sartorius stedim biotech, a leading international supplier for the biopharmaceutical industry today announced the launch of the biostat rm tx singleuse bioreactor, a. It is used with singleuse flexsafe rm bags which are proven for a broad range of different cell lines incl. It is based on a wellknown rocking technology providing mixing and aeration to the culture, which is maintained in an inflated, disposable cellbag bioreactor cultivation chamber.
Scaleup without tedious sequential transfers using singleuse cellbag bioreactors that can accommodate working volumes up to 25 l, ensuring an entire therapeutic dose can be obtained in a single bioreactor. The readytoprocess wave 25 bioreactor system was developed with the goal of keeping the simplicity of singleuse rocking. The ge wave bioreactor 2050eh is a flexible system built for use in research and development and the pharmaceutical production arena. Readytoprocess wave 25 bioreactor system end run harvest cytiva formerly ge healthcare life sciences. Part component description 1 uninet9port powerconnectiontotherocker. The system is based on a wellknown rocking technology that provides mixing and aeration to the culture, which is maintained in an inflated, disposable cellbag bioreactor cultivation chamber. This bioreactor can be expanded and reduced depending on your specific needs, quickly and ea. The rocker base of this bioreactor can be used with 20 liter wave cell bags where they are agitated, mixed with o2 and or co2. Introduction adenovirus is one of the most frequently used viral vectors in. Parallel processing, precise control of all relevant parameters, userdefined profiles, and innumerable automation features result in accelerated and highly efficient process development.
Readytoprocess wave 25 bioreactor system and the wave bioreactor 2050 system is shown. A bioreactor controller is used to measure and control process variables temperature, ph, dissolved oxygen, stirrer speed, and so on within a bioreactor vessel such as a glass autoclavable bioreactor, a single use bioreactor, or a single use wave bag. Ge readytoprocess wave 25 operating instructions manual pdf. Wave biotech bioreactor 2050eh inquire about availability system includes. Easy to use readytoprocess wave 25 system is designed for ease of use. The scope of this work was to exemplify the technical feasibility of the method editor and the dual functionality of the bioreactor system.
Wave bioreactor 210 and 2050 systems fig 1 are part of ge healthcare. Readytoprocess wave 25 bioreactor system introduction. Readytoprocess wave 25 provides users a peace of mind that allows them to reliably attend to other tasks. Optimization of microcarrierbased virus production in. Cryoprotectant was added to the culture and cells were cryopreserved in 4.
The biostat rm rocking motion, is a fully gmp compliant, wavemixed bioreactor. The readytoprocess wave 25 rocker features integrated temperature. The system can automatically determine suitable parameters for a run, a feature that facilitates fast start. Cellbag bioreactor attachment is straightforward and system. The singleuse readytoprocess wave 25 bioreactor system is a reliable and intuitive cell culture device for working volumes up to 25 l. The readytoprocess wave 25 rocker features integrated temperature sensors and load cells, designed for convenient handling and control of cell cultures up to 25 l. Dec 10, 2014 running the system with real time run status monitoring of cell culture conditions.
The wave biotech wave bioreactor system 20p is a cell bag bioreactor designed for routine cell culture. Demonstrating how to quickly start up a cell culture run with the system. Running the system with real time run status monitoring of cell culture conditions. Sartorius launches biostat rm tx singleuse bioreactor for. Wave bioreactor 2050 system the wave bioreactor 2050 system fig 4 is modular, consisting of an electrically powered base unit base2050eht and a choice of kits for holding the cellbag bioreactor. Our favorite innovative uses of the wave bioreactor technology, cell culture dish. Cultivation of t cells in suspension using wave bioreactor systems 210 and 2050 cultivation of e.
Ge readytoprocess wave 25 rocker cellbag bioreactor perfusion culture fermentor product id. Documents document view page ge wave bioreactor 210. Seamless transfer to pilot and production scale bioreactors are ensured by sartorius thorough understanding of bioreactor design and scaleup principles, wellthoughtout automation concepts and harmonized control strategies for oxygen, ph, temperature and feed addition. Readytoprocess wave 25 system components the readytoprocess wave 25 system consists of a rocker, a gas mixer, and a pump, all operated by the unicorn software installed on a client computer. Readytoprocess wave 25 system handbook 29009598 ac. Learn how this upgrade to the wave platform offers greater control over rocking rate, angle and acceleration. A general understanding of bioreactor systems and their operation is important prior to using the system for the first time. If no clear wave appears inside the cellbag bioreactor, change the rocking speed. This bioreactor system mixes and aerates cell cultures in up to 25 l volumes and combines an effective rocking technology with intelligent and. Data file 29061852 aa cell expansion ge healthcare life sciences xuri cell expansion system w25 the singleuse xuri cell expansion system w25 is a reliable and intuitive cell expansion device for working volumes up to 25 l fig 1. Current miniperfusion bioreactor was used for culturing initial seeded cells and optimizing the culture medium figure 3. More information is available on the readytoprocess wave 25 product page. Data file 29056695 ad readytoprocess readytoprocess wave 25 the singleuse readytoprocess wave 25 bioreactor system is a reliable and intuitive cell culture device for working volumes up to 25 l fig 1. The system is based on the wellknown wave rocking technology that provides mixing and aeration to the.
Dec 10, 2014 overview of the readytoprocess wave 25 bioreactor system. Cytiva standard software enduser license agreement for life sciences software productsreadytoprocess wave 25 system pdf microsoft software license terms windows embedded standard 7 pdf learn how the readytoprocess wave 25 bioreactor was used in scalable adcf virus production using hyclone vaccinexpress medium. New wave 25 bioreactor cell culture system from ge. Its modular system consists of a base unit and various integral options suitable for the culture of multiple cell lines. A reusable wave shaken polycarbonate bioreactor for protein production in insect cells achieves protein yields comparable to disposable bags. Data file 29061852 aa cell expansion xuri cell expansion. Wave bioreactor system 2050 eht used lab equipment. One vial from the highdensity cell bank was sufficient for direct inoculation of a bioreactor. Wave biotech products can be found through online auction and classified ads on labx. Ge readytoprocess cbcu bioreactor system by ge healthcare life sciences. In this video interview neil ross, ge healthcare, presents the new wave 25 bioreactor system. The high oxygen supply capability of the wave bioreactor system makes it ideal for insect cell culture. View online or download ge readytoprocess wave 25 operating instructions manual.
Bioreactor with a new innovative concept for all types of cell cultures on laboratory scale from 35 ml to 6 l culture volumes. Before installing and using the pump, please read through these instructions to familiarise yourself with the correct operation of the device. When at high cell density, the speed might need to be as high as 25 rpm. Track page views with auctivas free counter 233150484380. Significant time savings with simplified cell culturing using readytoprocess wave 25 with todays strict time frames, the demands for automation and ease of use in bioprocessing are rapidly increasing. Readytoprocess wave 25 rocker cytiva, formerly ge healthcare. Follow the manufacturers instructions on preparing the microcarriers. Wave bioreactor 210 system can be used with the following cellbag volumes. View and download ge readytoprocess wave 25 operating instructions manual online. Documents document view page ge wave bioreactor 210 20. Overview of the readytoprocess wave 25 bioreactor system. Readytoprocess disposable cellbag bioreactors for wave. You can rest assured that when you see the equipnet certified logo on a listing that you will be buying equipment that has been inspected, tested, and in many cases fully refurbished.
Wave bioreactor systems produce a very lowshear environment while maintaining mixing and oxygenation, they are ideal for the use of microcarriers. A bioreactor controller is used to measure and control process variables temperature, ph, dissolved oxygen, stirrer speed, and so on within a bioreactor vessel such as a glass autoclavable bioreactor, a. The wave 2050eht bioreactor system is a complete system and ideal choice for cell culturing, vaccination production, protein manufacturing, cho cells and so much more. Biotech wave bioreactor system base2e rocker works well. Readytoprocess wave 25 bioreactor system run youtube. Find laboratory fermenters, bioreactors and other laboratory equipment for sale and auction at labx. It is based on a wellknown rocking technology providing mixing and aeration to the culture, which is maintained in an. Fermenters and bioreactors new and used lab fermenters. Ge healthcare 28988000 wave25 readytoprocess rocker. Readytoprocess wave 25 ge healthcare life sciences. Readytoprocess wave 25 bioreactor system bioprocess online.
Optimizing process control in wave bioreactor 2050 system. Ge wave bioreactor 210 2050 systems operating manual this is the document view page. Wave bioreactor system 2050 eht the lab world group. Xuri cell expansion system w25 user manual 29064622 aa. Adenovirus production in singleuse readytoprocess wave 25. The rocker is the main unit and is used together with a tray and a disposable cellbag bioreactor. This not only reduces the risk of contamination and operator variability but frees up your time and work. Technicians evaluation the powered wave 25 rocker responds with a blinking green light, all buttons respond, and fan spins. Xuri cell expansion system w25 cytiva, formerly ge. Onestep seed culture expansion from one vial of highdensity.
Amprotein manufactured a current perfusion bioreactor system and singleuse bioreactor bags and columns inhouse through its bioreactor production platform and bag manufacturing apparatus photo 2. A reusable wave bioreactor for protein production in. It provides gas to the culture, and monitors ph andor dissolved oxygen. The results demonstrate a reproducible and robust batch production process for adenovirus using the readytoprocess wave 25 bioreactor system in 10 l scale. Wave bioreactor systems ge healthcare life sciences. Highdensity cell banks were prepared by culturing cho cells in the readytoprocess wave 25 system. The wave bioreactor system produces viruses under complete containment without the need for biosafety cabinet. Ge readytoprocess wave 25 manuals manuals and user guides for ge readytoprocess wave 25. Readytoprocess wave 25 bioreactor system from ge healthcare. Triplicate cultures demonstrate a reproducible and robust batch production of adenovirus.
Technicians evaluation the powered wave25 rocker responds with a blinking green light, all buttons respond, and fan spins. This bioreactor can be expanded and reduced depending on your specific needs, quickly and easily. Oct 18, 20 the singleuse readytoprocess wave 25 bioreactor system is a reliable and intuitive cell culture device for working volumes up to 25 l. Readytoprocess wave 25 the singleuse readytoprocess wave 25 bioreactor system is a reliable and intuitive cell culture device for working volumes up to 25 l fig 1. Montgomery county community college document number. Fin your ad below and contact the seller directly or request a quote.
Wave bioreactor base20eh system 20e rocker used working, 90 day warranty. Cbcu gas mixer is required for readytoprocess wave 25. Results obtained in this study, using the readytoprocess wave 25. Perfusion culture made easy with wave bioreactor 210 system duration.
Ge healthcare life sciences data file 29061852 aa cell expansion xuri cell expansion system w25 the singleuse xuri cell expansion system w25 is a reliable and intuitive cell expansion device for working volumes up to 25 l fig 1. Our favorite innovative uses of the wave bioreactor. The cbcu is a component of the readytoprocess wave 25 bioreactor system. Eshop for laboratory peristaltic pumps, syringe pumps and powder dosing instrument. Inspected, guaranteed, warranted and refurbished equipment. Operating mode with one cellbag bioreactor on the rocker. To maximize the window of operation, the effects of bioreactor rocking speed, angle, and motion on cell growth and viability were investigated in different culture volumes. Measure the temperature of the ph buffer standards using a thermometer. Readytoprocess wave 25 laboratory equipment pdf manual download. Read and understand this users guide before operating. Cell culture was conducted in cellbag bioreactors manufactured from either bioclear 11 or fortem films. The software platform, unicorn, allows for data monitoring and preprogrammed cell culture methods. We have 1 ge readytoprocess wave 25 manual available for free pdf download. Adenovirus production in singleuse readytoprocess wave.